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I believe API's CO2 booster is just gluteraldehyde, same as seachem excel. You can dose whenever you wish but the plants won't be able to use it until the lights are on. Same as the product doesn't start to degrade until it comes in contact with light. I just dose excel in the mornings before I leave (6:30am) and the lights don't come on till 2:00pm.
 
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