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I almost pulled that same mistake about 3 weeks ago when my wife stuck all her baby guppies in my nano tank... I had the co2 up like crazy. About 5 minutes later the guppies were all floating at the top gasping for air lol. Luckily I caught it and cranked the co2 down and stuck the air pump in there... managed to not lose any. It sucks, but live and learn!
 
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