Think I'd do it all at once.
Problem with this way is most bacteria live in substrate, so it might be hard to not get ammonia spike. On the other hand - it's much easier to do and you can just check your ammonia every day and if there's trouble do a big water change. Doing substrate change in parts will be more stressful for fish. So probably - do it all at once, after that big water change, like 60%. Check your ammonia and be ready to react.Think I'd do it all at once.
Okay so I've decided to stop fighting my substrate and I want to replace it. I don't want to start over for sure. My target substrate is going to be black blasting sand medium grit and I'm replacing my Safe-T-Sorb. I have about 2+ inches of the STS and I want to know if anyone has done a successful substrate change without completely draining the tank.
It is sucking micro nutrients from the substrate that I am bottoming out. I confirmed using my photometer (Hanna HI 83200) that my Manganese levels bottom out if I don't dose everyday. I don't know what else is bottoming out. Based on how I was able to grow my Tonina Fluviatilis with no issues after updating my micro dose means it was a micro deficiency issue.I'm curious what you're fighting as I've been thinking about using Saf-T-Sorb in a tank.