I dose 2 drops per gallon of water for every gallon of water change (the 100 ml bottle had a dropper cap, and I switched it to my 250 ml bottle). When I have done a 19:1 bleach dip for certain plants when algae got the better of me, I neutralized the plants by setting them in a bucket of tap water with a 10x the recommended Prime dose for 10m or so. Otherwise, 2 drops per gallon of Prime has never harmed my fish, and even when I completely forgot and added the normal amount of Prime an hour or two later, fish were fine.
Also remember that Prime neutralizes chlorine and chloramine for about 24 hours. Normal chlorines break down after about that time.