Nope, not stacked, I took the photo in-situ. You're right in that shooting at F22 provided good depth of field, but I was a little concerned about the loss of resolution in having the lens stopped right down. This critter stayed still long enough for me to shoot it at F11, F16 and F22. The F11 shot definitely had better resolution, but I decided that the depth of field was more important. If the critter had really fine detail I wanted to capture (like faceted eyes) I probably would have gone with F11. As it was, I learned a new Photoshop sharpening technique that improved the sharpness greatly. The problem was that it also created a lot of noise in the out of focus background. So then I ran the photo through Noiseware and voila!