On the last wash of new substrate I add a conditioner and nitrifying bacteria to the wet substrate before adding it to the tank. Am I overdoing it or should I carry on with my routine?
Since the organisms we are concerned with grow in a bio film, firmly stuck to surfaces, simply rinsing a cycled sponge is not going to wash off very many of the organisms. Better to move the sponge over to the new tank.running old sponge filter handy to rinse into the tank
Actually, they are still researching just which organisms are the ammonia oxidizing ones. Might not be an actual bacteria at all.The nitrifying bacteria you want are Nitrosomonas (ammonia-oxidizers)
Hmm, I will look more into that. Won't purchase the full document though haha. But from a glance, it seems that Archaea may oxidize ammonia, but I don't think that is present, or at least a dominant ammonia-oxidizer in freshwater aquaria.