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As long as you keep the substrate wet, and the humidity up so they don't die in the mean time, the should root. I bought a bunch of MC like that. And it ended up in an emersed tank rather than the desired aquarium because of no roots. It's growing well now. Granted, still not in the desired tank, so using dsm in target tank you *should* be okay. Just do not let them dry out.

What happened is they probably did not change their growth regulators in the tissue culture media. If there is adequate carbon in it still, and depending on the plant growth regulators in it, they won't grow roots at all, until the media is becoming depleted.

It's great when multiplying them, they spend zero energy on growing roots you're gonna cut off. But if they don't change the media composition for sale packaging, you end up with that still. It's a hard balance making sure they will grow to nice size in those cups and still grow roots once you transfer from multiplication containers to sale ones. Plants grow roots to seek nutrients. In tc plants, you give them carbon (can grow in dark, don't need to make sugar since you give to them). You give all macro/micro nutrients with basal salts and other supplement in media. You give the growth regulators to override what the plant is producing in it's meristems and make it grow like you want it to. Want no roots? Np. Want no shoot growth? Np. Want to make them grow as undifferentiated callus and create somatic embryos? Np.

Now that gel is gone, the plant will rely on its own growth regulators. Or lack thereof. It's likely not producing much since the media was. In the lack of any regulators added to media, they'll grow roots (or with different mix of regulators but still adding them). Itll realize there's no more supply of everything and start to compensate on it's own. As long as they stay in very very high humidity and on wet substation, you'll get roots. Just keep that lid closed so they don't dry out

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Really thorough explanation. @adkaquascaping I enjoyed it.

Also, OP, did you mean hemianthus callitrichoides ? H. Micranthemoides is pearlweed I believe. Grows taller than HC.
Lack of rooting is why ive been tweaking my own rooting media to find out how low I have to cut nutrients. And gotta make sure you don't use too much agar. Most of them do not like media that's nearly as solid. I've had E. parvula (dwarf hair grass) refuse to grow runners, and only grow roots between agar and container walls because agar was too firm. Hah. Darn things. Picky AF. I thought I'd just transfer techniques from terrestrial and run with it. Nope. Gotta optimize each phase of media for each plant. Far more work starting up than I anticipated haha. I love it though
 

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It's been many years since I've done this stuff but the basics haven't changed much. Beyond standard nutrients adding auxins to your media will greatly stimulate root growth. Kinetin/cytokinins will stimulate more vegetative growth but roots usually are more what you want for propagation purposes. Also for more root growth typically you'd want to go with a softer agar say 1/2-1/4 normal. As you note, the specifics need to be worked out for particular plants but using phytohormones as above will let you better tailor the growth. Plate up a range of concentrations to see what works best and then go from there to better optimize.

I've not tried to use them for aquarium plants but there are off-the-shelf rooting compounds that you can get easily if you don't have access to lab grade products which should work well for DSM use, e.g., https://www.amazon.com/Miracle-Gro-...=UTF8&qid=1540367419&sr=1-1&keywords=fastroot
Access it's no issue. I cashed in out my retirement to build a lab. I went to college for biotech, ended up in semiconductors... So i decided to build my own company from the ground up :) I worked on the American Chestnut Project in college. Done everything from plasmid design and putting plant genes into bacteria, to using bacteria to put genes into plants, tissue culture work (from embryo rescue right on through to hardening off plantlets).


All very solid info tho.

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