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Hemianthus callitrichoides - Experimental Growth

2922 Views 10 Replies 5 Participants Last post by  Mike A.
It all happened by accident.

I purchased 2 tubs of In-Vitro HC 'Cuba' from Hortlabs. I am currently starting a Nano Tank using the DSM and I've been dying to try HC for a long time now. When the tubs arrived, I was floored at the superb condition these plants where. Amazing, but I was about to receive a shock when I prepared these for planting.

Instructions state, remove from container, clean off Gel and plant (roots down) into the substrate. Easy enough. I removed the first HC Puck, from the Gel, flipped it over and was shocked to see --- NO ROOTS. Seriously, not one.

How am I going to plant this? Then a simple experiment from Grade School came into mind, the seed and wet paper towel. The seed was able to grow in the paper towel fine. The root grew horizontal in the same orientation of the seed. Then you plant it root down.

So, by science and by gravity, simply placing these onto the substrate (giving them a little press with my thumb), these should develop roots and anchor themselves into the substrate.

I cleaned up the pucks, cut them up and had more than enough to place all over my substrate. I've looked all over the Internet a few people have grown this plant on Driftwood and rocks. Some reports I question, others I know to be 100% genuine (James Findlay being one of them).

So I've decided to run this experiment to see if this will actually work. If it doesn't work, Iwill purchase more that have visible root systems from somewhere else. If it works, I found a relatively painless way to get HC to root in an Aquarium using the Dry Start Method (DSM).

Soil is moist, and the humidity is at 75%.

If anyone has information regarding this, please chime in. I would love to hear views on this.

Gary
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Lack of rooting is why ive been tweaking my own rooting media to find out how low I have to cut nutrients. And gotta make sure you don't use too much agar. Most of them do not like media that's nearly as solid. I've had E. parvula (dwarf hair grass) refuse to grow runners, and only grow roots between agar and container walls because agar was too firm. Hah. Darn things. Picky AF. I thought I'd just transfer techniques from terrestrial and run with it. Nope. Gotta optimize each phase of media for each plant. Far more work starting up than I anticipated haha. I love it though
It's been many years since I've done this stuff but the basics haven't changed much. Beyond standard nutrients adding auxins to your media will greatly stimulate root growth. Kinetin/cytokinins will stimulate more vegetative growth but roots usually are more what you want for propagation purposes. Also for more root growth typically you'd want to go with a softer agar say 1/2-1/4 normal. As you note, the specifics need to be worked out for particular plants but using phytohormones as above will let you better tailor the growth. Plate up a range of concentrations to see what works best and then go from there to better optimize.

I've not tried to use them for aquarium plants but there are off-the-shelf rooting compounds that you can get easily if you don't have access to lab grade products which should work well for DSM use, e.g., https://www.amazon.com/Miracle-Gro-...=UTF8&qid=1540367419&sr=1-1&keywords=fastroot
 

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Access it's no issue. I cashed in out my retirement to build a lab. I went to college for biotech, ended up in semiconductors... So i decided to build my own company from the ground up :) I worked on the American Chestnut Project in college. Done everything from plasmid design and putting plant genes into bacteria, to using bacteria to put genes into plants, tissue culture work (from embryo rescue right on through to hardening off plantlets).

All very solid info tho.

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Ahhh... My background is similar. Worked a lot with cellular-level hybridization and culture of resulting lines through full plants. Later worked at a commercial operation for a while. Then also went over to the tech dark side. You're in good shape then. You have what you need to do it right and know what you're up against as far as contamination, etc. I see people here and elsewhere trying to culture things at home all the time. Pretty much know how that's going to turn out before they even start. ; )

Now just go create an algae-resistant low-light carpeting hybrid and you'll be rich. ; ) Good luck with the company!
 
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