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Only use the bubbles per second to calibrate yourself. To determine your max CO2, slowly turn up the CO2 until you see negative signs from your fish, and then turn it down slightly. Now look at your bubble count, and that is where you want to be in the future.

By slowly, I mean a very slight (1/16-1/8 turn) adjustment in increased bps, and wait an hour or so. If no negative signs, adjust it up slightly more, and so on.

Your bps will depend on your flow, type of filtration, surface agitation, CO2 dilution, working pressure, and likely much more. Its not a reliable way to compare between tanks.

So, in a nutshell, you guys are saying if CO2, then no Excel needed. Fair enough. My next question. On my 90g tank, how many bubbles per second should I aim for. I'm using an inline reactor and seeing almost no micro bubbles.
 
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