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I ran a DIY system my 55 gal and never ran an airstone at night. How many bottles are you running on your 20 gal? I would run 1 bottle with the line running to a Rex Style Reactor on that setup and let it run 24 hours in the tank with no airstone and your fish should be fine. I ran 2 bottles on my 55 and here are my results with no fish deaths...

 
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