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I was wondering if you had a high density of tubifex worms, like approaching the sickening density of those sewer slime monster videos, then would their respiration result in CO2? Also, consider that I have an undergravel filter attached to a powerhead. I'm thinking the water being pulled down will oxygenate the tubifex worms, causing them to release CO2. Is that feasible?

Also, I encountered this thread on CO2 generating substrate recipes:
Cheap Sand-based CO2 Generating Sub

The guy says he has tubifex worms, but that's not where the CO2 comes from. Also, he doesn't have an undergravel filter. What generates the CO2 are marble chips in the substrate. I'm thinking that the additional flow of an undergravel filter would cause the marble to deteriorate slightly faster? Does this person's idea even make sense? Do marble chips release CO2?

BTW, I currently have blackworms in the substrate. They don't seem to produce enough CO2 to promote plant growth. I'm guessing the tubifex worms can breed more densely, enough to become those disgusting sewer masses. If your substrate was half gravel/sand, half tubifex worms, with fresh water constantly being pulled downward through it, would their respiration contribute a significant amount of CO2?
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