1. I will set up the 20 gal half full of pre mixed saltwater and adjust to 34ppt salinity using R/O water to reduce salinity or aquarium salt to increase salinity. The put the light against the side of the tank and leave it on 24/7 to promote algae growth for the zeo's to feed off.
Aquarium salt and Marine or Reef salt are not the same things... if the salinity is too low, you need Marine or Reef salt to up the salinity.
You may want to look into some phytoplankton or other small foods to feed the zoes, too....
2. Once I can see tiny eyes in the eggs of the buried female I will move her to the 1 gal container and fill with aquarium water from the tank she came. I will add an air stone with very low flow and feed 1 spirlina flake daily until she releases the zeo's. If water changes become necessary I will remove 25% and add more tank water.
Moving a berried amano into another container may cause her to drop her eggs... it can take anywhere from 15-50 days for the eggs to hatch.
3. Once she releases the zeo's I will then remove the female and put her back into my main tank.
Amano eggs can hatch over a period of days, if not weeks... be sure to have a flashlight on hand to use on the side of the aquarium to check for babies when it's completely dark!
4. I will then fill another 1gal tupperware container with saltwater from the 20gal tank that has been aging and growing algae. Using an air stone to prevent sucking up the zeo's I will siphon 1/2 of the water out of the container with the zeo's and start a dip of saltwater to top it off. I will be trying a drip ever 5 sec or so.
You could honestly just dump the zoes straight into saltwater, or even brackish, within a week's time of hatching and they'd still be okay...
The zoes are *normally* born in freshwater streams or rivers which lead out to the ocean. They tend to unceremoniously get "dumped" into saltier conditions...
5. Once the acclimation to partial saltwater is done I will float the one gal container for about 2 hrs in the 20 gal to ensure a slow temp acclimation. I will be trying a temp of about 70F for the zeo's.
6. From here on out I will be monitoring the water for clarity and feeding as necessary as well as testing the parameters daily. I chose a 20 gal for this purpose as I do not plan on water changes but will do if needed.
Rather large tank. Good thought process!
7. Once I see forward swimming shrimp (zeo's that have morphed) I will wait 2 days and then start the acclimation back to freshwater over a 1 day period.
It can take 3-6 weeks for the zoes to morph into miniature adults... so you can't acclimate the entire tank at once to freshwater... sorry, not that simple! Also, you might kill the new shrimp doing an acclimation to freshwater that fast.
A day after you start noticing the miniature adults (aka, when the forward moving babies are no longer red), you then catch them, put them into a container and do 50% water changes with freshwater. If you want, you can take out 50% and drip freshwater back into their container. Do this for 3 days.
Day 1 morph - Saltwater Tank
Day 2 morph - Saltwater container with 50% water change (if you start with 34 ppt, it will be 17ppt)
Day 3 morph - Brackish container with 50% water change (~8.5 ppt)
Day 4 morph - Salty Freshwater with 50% water change (end up being ~4 ppt)
Day 5 morph - Freshwater
Adults cannot live in full marine conditions. They can, however, live in brackish water, no more than 17 ppt. (not recommending it, though!) Although the zoes can live in full marine conditions (34 ppt), the newly morphed young cannot survive lone in marine conditions either... and transitioning them to freshwater over a period of 1 day may be too big of a change and kill them.
8. My goal is to have 40+ Amano's make it to maturity and work on improving yields in the future.
Amanos can have a minimum of 300-400 offspring, and potentially over 2,000... (may depend on age of amano) So if you can even get 1 to survive, that would be an accomplishment!
My best attempt, which was my first, the longest I had one survive was up to 2 weeks in saltwater... if your first attempt fails, you might try doing brackish. Some people have better luck in saltwater, where-as others have better luck in brackish water...