I have used the product 3 or 4 times with my own and another person's tanks and have not observed instantaneous cycling, but it never took more than 48 hours. Of course, that was early 2004, before the product became temporarily unavailable.
On the occasion when I added the bacteria and fish at the same time to a new tank, I used only half the biospira package, then added the other half the next day, after the fish had a chance to produce waste. Because I don't know the lifespan of a Nitrospira bacterium in 75-80 degree water with no food source, I cannot say whether that was necessary or not.
Tank: 40 Gallon Breeder w/Eco Complete ||
Aquasun 156W Quad 5000K || SunSun 302 Canister Filter (Sunsun Pimp #77) || Pressurized CO2 || Dual Current Gamma 15W UV Sterilizers || Hydor Inline Heater
Last edited by EricSilver; 03-23-2005 at 06:14 PM.