Research Proposal Ideas....Need some ideas!! - Page 2
 Your Tanks Image Hosting *Tank Tracker * Plant Profiles Fish Profiles Planted Tank Guide Photo Gallery Articles

 The Planted Tank Forum Research Proposal Ideas....Need some ideas!!
 Register FAQ Members List Search Today's Posts Mark Forums Read

 02-14-2014, 02:16 PM #16 Raymond S. Planted Tank Guru     PTrader: (5/100%) Join Date: Dec 2012 Location: Hot Springs Ar. 71901 Posts: 4,722 You would do yourself a favor to ask someone who is qualified to answer this question...how much does this gas weigh compared to regular air. Some gasses are lighter and some are heavier. Displacing the air with a heavier than air gas would be easy. If it's lighter than air you will need to find another way other than pressure. Those tanks can't take even 2 Psi. Just putting a piece of banana in there with the top sealed except for a small opening would displace the air if the gas is heavier. __________________ The shortest distance between any two points is a straight line...in the opposite direction...

 02-14-2014, 02:33 PM #17 THE V Planted Tank Enthusiast     PTrader: (0/0%) Join Date: Nov 2011 Location: California Posts: 521 C2H4 = 28, CO2=28 so pretty much the same as air. Ethylene is a growth regulator and takes tiny amounts to have major effects. ppm and ppb is common.
 02-14-2014, 10:23 PM #18 lochaber Planted Tank Guru     PTrader: (1/100%) Join Date: Jan 2012 Location: Oakland, CA Posts: 2,318 Most of our atmosphere is N2, then O2, with a tiny bit of Ar. CO2 is only a fraction of a %, and it's molecular weight is ~44 - I think you omitted an oxygen, THE V
 02-15-2014, 12:58 AM #19 THE V Planted Tank Enthusiast     PTrader: (0/0%) Join Date: Nov 2011 Location: California Posts: 521 Can't do math in the morning apparently. Sheesh
02-15-2014, 08:27 AM   #20
plantbrain
Planted Tank Guru

Join Date: Dec 2003
Location: The swamp
Posts: 13,562

Quote:
 Originally Posted by THE V Question: Would increased ethylene concentration prior to flooding in a DSM cause a faster adaption to submerged growth? i.e. Put a bunch of banana's in the tank and seal the top for a week prior to flooding. We'll have to call it "Feeding the Monkey". LOL
I would hypothesize that.
But what would you suggest be the metrics for adaptations to submersed growth? Plant species etc?

As should be the case, one question leads to several more.
__________________
Regards,
Tom Barr

02-15-2014, 08:46 AM   #21
plantbrain
Planted Tank Guru

Join Date: Dec 2003
Location: The swamp
Posts: 13,562

Quote:
 Originally Posted by clincoln9 These ideas are quite intriguing. I could certainly do a basic DNA electrophoresis in agarose or polyacrylamide gel, but then trying to transform the red from an RCS to an amano, that I'm not sure is something I could pull off...that would be incredible though.
Well, you can see what research has been done on an aquatic plant or a shrimp etc that has some phenotype they you can see. I agree it would be hard and while you might not answer that specific color gene isolation, you might find many other things that are of interest and different between the wild type and the Red. You try something and then see what you get. Maybe you get lucky, maybe you do not get what you want,m but you get a group of genes that codes for a mutant enzyme perhaps that confers color. Conformation it would be harder. But I think the 1st step is trying and then seeing what you can get out of that. Then go from there if you have time.
Depends on how much you are into molecular side of things and if you could parlay this into something more with an eye towards grad school. also depends if there's a lab that has some resources and can help you better focus your ideas and goals. I would go talk to a prof about it and see what ideas you can both come up with. They like students who come asking good basic questions.

Quote:
 As for the anthryocyain production in red plants with the presence of jasmonic acid, that also seems like it could be an interesting project. I was curious so I read a few experiments done in a similar manor with terrestrial plants. I read that anthocyanins have been found to inhibit growth of cancer cells...how has more research not been done on this??? Basically the problems I read were that there was no commercially viable process to grow and extract the anthocyanins. One study found jasmonic acid and intense light to be best for production. Could be fun to test this with aquatic red plants!
There's a practical side to this. JA is a chemical you could add to increase red coloration in aquatic plants. I looked into using it in a trace fertilizer mix, but it's not cheap. Then would anyone buy it and at what price in this small aquarium sub hobby?

Not a wise business move really.

But for research and cancer? Then it becomes a much better method. AC's can be extracted via chromatography.
eg TLC:

http://bipublication.com/files/IJABR-V3I3-2012-09.pdf

You can certainly do it.

Quote:
 Does that mean if we could induce this while still emersed(not sure how that could be done), then by the time of flooding, the plant would be more ready for slower gas diffusion that occurs in water?
See DSM for plants.
You could as suggested by V, to do a dry start and in one tank add Ethylene.
This does not need to be a high tech thing, just a source of the gas, and have a little bit leak out and have the gas added for say a few days etc.
You can use small glad tupperware like food containers and soil to do the emergent growth and have plenty of replications. Then flooding them in an aquarium.

Ethylene treated vs non Ethylene treated.

You could pick red Luwigia(which will turn green in emergent growth, but red submersed). This might take 3-4 weeks to run this test, you could measure the AC content via TLC also.

You could use dry weight comparisons for growth.

That's all doable in a semester. I'd likely do this if I were you.
__________________
Regards,
Tom Barr

Message:
Options

## Register Now

In order to be able to post messages on the The Planted Tank Forum forums, you must first register.
User Name:
Location
 Your Location. As precise as you feel comfortable with.
Security Question
 While balancing on a piece of wood, two inches by four inches known as a 2x4, John and his friend Sally both spotted a dalmatian inside of a truck with sirens. The animal with John and Sally is a _ _ _?
Insurance

## Log-in

Human Verification

In order to verify that you are a human and not a spam bot, please enter the answer into the following box below based on the instructions contained in the graphic.

 Thread Tools Display Modes Linear Mode

 Posting Rules You may post new threads You may post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is Off Forum Rules
 Forum Jump User Control Panel Private Messages Subscriptions Who's Online Search Forums Forums Home The Planted Tank Information     Important Update/Announcements General Planted Tank Forums     General Planted Tank Discussion         Herp (Reptiles and Amphibians)         Ponds         The Planted Tank FAQ         Riparium/Terrarium/Vivarium     Local Pet Stores, Clubs, and Conventions         Alabama         Alaska         Arizona         Arkansas         California         Canada         Colorado         Connecticut         Delaware         Florida         Georgia         Hawaii         Idaho         Illinois         Indiana         Iowa         Kansas         Kentucky         Louisiana         Maine         Maryland         Massachusetts         Michigan         Minnesota         Mississippi         Missouri         Montana         Nebraska         Nevada         New Hampshire         New Jersey         New Mexico         New York         North Carolina         North Dakota         Ohio         Oklahoma         Oregon         Pennsylvania         Rhode Island         South Carolina         South Dakota         Tennessee         Texas         UK         Utah         Vermont         Virginia         Washington         West Virginia         Wisconsin         Wyoming     Low Tech Forum     Planted Nano Tanks     Tank Journals     Photography Specific Aspects of a Planted Tank     Equipment     Lighting     Substrate     Aquascaping     Fertilizers and Water Parameters     Algae     Fish     Shrimp & Other Invertebrates     Plants     DIY General Forums     The Lounge & Introductions     Other Websites Sponsors     ACUREL Aquarium and Pond Care     Aquascape Depot     Aqua Design Innovations     Aquatic Garden     Aquatic Gardeners Association     Aqua Forest Aquarium     Aquatek of California     Aquatic Foods-California Blackworm Co.     AQUATOP Aquatic Supplies - Planted Aquaria     Aquacave     Aquasafe Water Filtration Systems     Benibachi USA     Build My LED     Current-USA and Ecoxotic     CO2Art Ltd     Finnex     Green Leaf Aquariums         Green Leaf Aquariums Archive     Invertebrates by Msjinkzd     LEDGroupBuy.com     Orphek     QShrimp     Ahquatics.com     Sevenports     Southern Oak Aquatics

All times are GMT. The time now is 10:21 AM.

 -- The Planted Tank -- PlantedTankLight Acceptable Use Policy - Advertising - Contact Us - The Planted Tank - Archive - Privacy Statement - Top