After just over 48 hours the Kh has gone up to just under 150, which is where it was prior to the water change. In preparation of moving some cherry shrimp into this tank, I moved the guppies into the sump. There are now about 14 of them in the 10 gallon sump. One Male fancy guppy and some fry, that weren't in there two days ago, were left in the main tank. I also changed the Ph meter to turn on the CO2 at 7.0. That will give me 25 ppm, which according to Chuck's calculator is high.
I can only find four ottos in there. Three of them always stick together, and one is a loner.