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You knock drip acclimation. So you went ahead & just added fish without quarantine. What did you expect. They had a disease & they wiped out your healthy batch. Your first batch should have been quarantined for 30 days as well. Drip acclimation is important especially if there is a TDS difference of more than 40 TDS. Otherwise the fish can die of ossmotic shock. The cells can swell & explode. It is the # 1 reason fish die when first introduced into aquariums. Drip acclimation over several hours can actually relieve stress on fish.