Planted Tank Guru
Join Date: Mar 2009
Location: Citrus County,Florida
Based on how this thread developed for me interest ended on 6/15/13.
This will be my final post to it.
Shawn, if you have no objections I think we should move the discussion over to your old thread and I'll cross post what matters there. (your call, let me know here or in PM)
In post#24 I mentioned taking more fish to the lab. I just received the path report and I'm posting it in it's entirety.
These fish arrived here mid year last year as specialty dime size angelfish. Showed little or no outward sign of defect and were roughly 1yr old. Myco was confirmed within the eight fish I received last December after only two losses to wasting and a third failing when tested. The full report was completed in the first quarter of 2013. I still had maintained four fish in a 90g closed loop tank hoping to confirm vertical transmission.
Identifying three as male and one female I had the two secondary males surveyed.
Report Status: Preliminary 0 Final 1
Fish A = Blue smoke; fish B = “glitter”
Fish A had 2 ulcerations approximately 5 mm in diameter on either side of the body and erythema and swelling of the base of the right pectoral fin. A scraping of one of the ulcers revealed a poorly organized granuloma and numerous white blood cells. Gill and fin biopsies were unremarkable.
Gross findings at necropsy revealed the lower intestine distended with fluid, though no parasites were observed microscopically. Numerous trichomonads were observed in an anterior feces-filled section of intestine. Numerous granulomas were observed in anterior kidney, spleen, and posterior kidney. The liver contained lipid droplets. No gonadal tissue was identified. Spleen and anterior kidney were cultured on tryptic soya agar (TSA) with 5% sheep blood, but no growth occurred.
Histopathology results: Skin-A moderate to severe inflammatory reaction including macrophages and lymphocytes was observed in the dermis, sometimes extending into the muscles, in the region of the ulcers.
Pectoral fin-inflammatory reaction including lymphocytes and macrophages.
Gill-mild to moderate telangiectasia, occasional hyperplasia and fusion; several lamellae enveloped in extensive inflammatory response including macrophages; extensive inflammatory reaction in branchial arch, including many macrophages and one granuloma.
Intestine-extensive eosinophilic granular cells (EGCs) in submucosa, infrequent foci of lymphocytes.
Liver- mild hepatic lipidosis.
Posterior kidney-extensive severe granulomatous disease acid-fast positive beaded rods observed; numerous hyaline droplets in ~50% tubular epithelium.
Anterior kidney- extensive severe granulomatous disease, approximately 75% of tissue is granulomas; acid-fast positive beaded rods observed.
Spleen- extensive severe granulomatous disease, approximately 90% of tissue is granulomas; occasional acid-fast positive beaded rods observed.
Brain-no significant findings.
Heart-very large coalescing granulomatous structure in ventricle; occasional beaded acid-fast positive rods observed.
Except for through and through ulcerations of the left side of the mouth, Fish B appeared normal. No pathogens were observed on biopsies of skin mucus, gill, and fin. At necropsy the liver of this fish was located in the right coelom, which is highly unusual for this species. Numerous granulomas were observed in spleen, anterior kidney, and light granulomas were present in posterior kidney. Testes was identified, and granulomas were observed within. The intestine appeared normal, but numerous trichomonads were observed. Spleen and anterior kidney were cultured on TSA with 5% sheep blood, and a gram-negative, cytochrome oxidase-positive bacillus (common aquatic environmental bacteria such as Aeromonas, Vibrio, and Pseudomonas and usually opportunistic) grew from both organs.
Histopathology results: Gill-moderate telangiectasia .
Branchial arch- large focus of inflammatory cells, lymphocytes and macrophages.
Mouth lesion- mild multifocal necrosis, moderate to severe inflammation.
Testes-one granuloma containing necrotic cellular debris and acid-fast positive bacteria.
Liver-one small basophilic focus and associated EGCs; one small granuloma that contains a few acid-fast positive bacteria; one focus of large adipocytes.
Heart- large focus of granulomas in ventricle with small groups of EGCs and three acid-fast positive rods; most of atrium is granulomatous inflammation and numerous EGCs.
Posterior kidney-approximately 40% of tissue is severe extensive granulomatous disease; numerous acid-fast positive bacteria observed; approximately 20% of tubular epithelium effaced by hyaline droplets.
Spleen- approximately 60% of tissue is severe extensive granulomatous disease; numerous acid-fast positive bacteria observed.
Anterior kidney- approximately 50% of tissue is severe extensive granulomatous disease; numerous acid-fast positive bacteria observed.
Brain-no significant findings.
Intestine-multifocal mild necrosis, occasional small clusters of EGCs; numerous bacteria of various shapes and sizes in the lumen along with plant material.
Acid-fast staining confirmed extensive, severe mycobacteriosis in both fish. We recommend humane euthanasia of remaining angels from this group. Currently, it is unclear if any of the other fish in the system are infected with Mycobacterium. Careful consideration should be given to the disinfection of the system/tank holding these fish.
Strongly consider what you are doing if you ignore myco after it's confirmed
Regards to all who have posted in this thread.
OP, plz clean the tank.
The Fraternity of Dirt
If at first you don't succeed,,, keep kicking it
2-75g planted, 5-55g planted, 5-20g planted, 110g w/30g sump, 8-10g,
2012 update adding table top pleco pans & a 90g (Nutz)