marko- I don't have a hardness testing kit, and I don't know that I'd trust the readings I would get on those test strips. pH is high out of my tap, but I use a Betta water conditioner with Indian Almond leaves and stuff in it to get the water to around 7 pH, a nice middle ground for all the species involved. Some would say neutral:p.
inka- Thanks, and I think it'll be even better once I get a trim done.
xenxes- I have read that 2g is too small, and were this a plant only bowl I would be worried, but there is a zoomed 501 pushing water around and producing a nice current and plenty of filtration. I have never found any measurable ammonia, nitrite, or nitrate.
I was more worried with the Otos(there were 3!) not having enough algae to eat, so I would give them blanched zucchini a couple times a week. The first died rather quickly, the second after about 2 months, and the last is looking full and frisky.