The Planted Tank Forum - View Single Post - Anyone with biology knowledge: boiling or baking to kill Mycobacteria/Fish TB?
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post #14 of (permalink) Old 02-14-2012, 01:23 PM
Algae Grower
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Join Date: Jan 2012
Location: Gainesville, Fl
Posts: 101
Unfortunately, heat as in boiling or baking won't work. You would have to autoclave everything (121.21 degrees celcius) for about 90 minutes to be 100% sure you killed it. If it forms spores, you will have to be careful about even using chemicals as some bacteria's spore can actually resist things such as bleach and alcohol. I know with bacillus bacteria they form spores and contaminate a lot of things in the micro lab I used to work in. If we heated a sample to less than 81 celcius, then bacillus would live in a spore form but everything else that was non-spore forming would die.
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