5/28/2006: Yet another cardinal spawn today! This is the largest one yet, as I was able to find about 20 to 30 eggs. I divided these among 4 separate vials in order to test various conditions as follows:
- Tank Water (220 ppm)
- Tank Water + Methylene Blue (220 ppm)
- Diluted Tank Water (120 ppm)
- Diluted Tank Water + Meth Blue (120 ppm)
The results? No hatchings in any of the vials! Arrgh.... There are a few possibilities I can think of.
- TDS of 120 ppm is still too high.
- Possibly overdosed Methylene Blue in the two vials.
- Fungus killed the eggs in the vials without Meth Blue.
- Eggs weren't fertile.
Of these possibilities, I feel the most likely are that the TDS is too high, or perhaps even more likely, that the eggs simply weren't fertile. I'll try the next spawn with even softer water to see what happens. Now if the eggs were infertile, this could be for a few reasons.
- Females were carrying old and/or calcified eggs as a result of not spawning for a while. If this is the case, then hopefully any new eggs generated will be healthy, now that these have been cleared out.
- Males need more practice. (Doubtful but possible)
- Males, and perhaps even females are beyond their breeding prime.
- Perhaps I have no males at all.
- Water parameters are not suitable for the sperm.
Ultimately I guess we'll just have to wait and see what happens.